As proof-of-principle, two target genes were edited with high efficiency in an EGFP-Cas9 stable CHSE cell line specifically, the exogenous, integrated EGFP and the endogenous RIG-I locus. In the current study, we developed an optimised protocol using lentivirus transduction for efficient integration of constructs into the genome of a Chinook salmon ( Oncorhynchus tshwaytcha) cell line (CHSE-214). However, use of genome editing to evaluate putative disease resistance genes in cell lines, and the use of genome-wide CRISPR screens is currently limited by a lack of available tools and techniques. Salmonids are the most important aquaculture species by value, and improving genetic resistance to infectious disease is a major goal. Genome editing is transforming bioscience research, but its application to non-model organisms, such as farmed animal species, requires optimisation.
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